integrin alpha v beta 6  (MedChemExpress)

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: (A) Flow cytometric analysis of POD 16-tolerant and CLAD allografts with (A) representative contour plots gated on live CD45-allograft cells (N=5/group) and (B) Intracellular Sftpc expression analysis on CLAD allograft CD326+ αvβ6+ cells. The histogram shown is representative of three independent experiments. (C) Plot of POD 16 allograft numbers of live CD45-CD326+ αvβ6+ cells shown with a mean ± standard deviation from the mean for N=5 per group. Representative immunohistochemical stains with αvβ6 specific antibodies with indicated magnification and scales (N≥4/group).

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Expressing, Standard Deviation, Immunohistochemical staining

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: αvβ6 immunohistological staining of allografts from recipients that underwent club cell injury and received (A) CCL2 neutralizing Abs, (B) CD8α depleting Abs, respectively, and (A, B) Control Ig injections followed by POD 16 [ 64 Cu]Cu-DOTA-A20-K16R PET/CT imaging. Images shown are representative of N≥4 per group. Scale bars: 400 μm for 10x, 100 μm for 40x.

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Staining, Control, Positron Emission Tomography-Computed Tomography, Imaging

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: (A, Left Panel) Representative autoradiographic images following incubation of [ 64 Cu]Cu-DOTA-A20-K16R with human explanted lung tissue sections from a patient with CLAD and Control lung tissue from a donor lung obtained prior to transplantation. Preincubation with an excess of a cold probe sharply reduces autoradiographic activity (Cold Probe/CLAD). (A, Right Panel) corresponding trichrome-stained lung sections that were incubated with radiotracer. Images are representative of independently conducted experiments, with (B) violin plots showing combined data from 3 explanted CLAD transplants with or without cold-probe blockade and 3 control lungs per group. Two-sided Mann-Whitney U t-test *p<0.05, ***p<0.001. Representative αvβ6 (C) immunohistochemical and (D) immunofluorescent staining of human explanted lungs with CLAD and control lungs (N=3/group). Arrows denote clusters of cells expressing αvβ6. Scale bars: 400 μm for 10x, 100 μm for 40x.

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Incubation, Control, Transplantation Assay, Activity Assay, Staining, MANN-WHITNEY, Immunohistochemical staining, Expressing

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) At the level of striated muscle fibers, where the cytoplasmic pattern predominated; (B) At the level of minor salivary glands, with cytoplasmic and membrane pattern, the reactivity being more evident in the serous acini; (C) At the level of hairs from the lips, with predominant cytoplasmic reactivity; (D) At the level of sebaceous and sweating glands from the lips, with predominant cytoplasmic and nuclear staining (the latter being more evident in the luminal cells of the sweat glands); (E) A weak reactivity observed at the level of the nerve threads; (F) At the level of inflammatory cells (especially in lymphocytes and plasma cells) and in the endothelium of blood vessels, with predominantly cytoplasmic reactivity. Anti-Integrin αvβ6 immunolabeling: (A–F) ×200

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Staining, Clinical Proteomics, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) At the level of normal epithelium, basal cells were not reactive or the cytoplasmic reactivity was weak, but the membrane pattern was especially present at the level of the upper rows of the spinous layer; (B) In low-grade dysplasia, we noticed the tendency to change the subcellular pattern from the membranous to the cytoplasmic to the upper rows of the spinous layer; (C) In moderate-grade dysplasia, the same trend was observed as in low-grade dysplasia, but in the inner two-thirds the reactivity was absent or present with a weak membranous pattern; (D) In high-grade dysplasia, we noticed a nuclear reactivity extended throughout the entire thickness of the epithelium and a weak cytoplasmic reactivity in the upper part of the epithelium; (E) In the epithelium with koilocytic atypia, the reactivity was present in the peripheral cytoplasm, being more evident in the upper layers; (F) In well-differentiated tumors, the prevailing pattern it was membranous especially inside the tumor islands at the level of neoplastic cells with morphology similar to normal keratinocytes. Anti-Integrin αvβ6 immunolabeling: (A–C, E and F) ×200; (D) ×100

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) In well-differentiated OSCC, the ortho- and parakeratotic pearls were negative; (B) In the moderately differentiated OSCC, the predominant reactivity is cytoplasmic, maintaining membrane reactivity only at the level of well-differentiated neoplastic cells; (C) In the less differentiated OSCC, cells lose their membrane reactivity and acquire a predominantly cytoplasmic and even nuclear pattern more evident towards the periphery of the proliferations and in the invasion front; (D) An intense membrane and cytoplasmic reactivity observed at the level of acantholytic neoplastic cells in those variants with extensive foci of acantholysis; (E) In the poorly differentiated OSCC, pattern of reactivity is the same as in moderately differentiated variant with an intensification of the nuclear staining; (F) Regardless of the degree of differentiation, the reactivity was more evident in the invasion front, the invasive islands having a predominantly cytoplasmic and nuclear pattern. Anti-Integrin αvβ6 immunolabeling: (A, C and F) ×100; (B, D and E) ×200. OSCC: Oral squamous cell carcinoma

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Variant Assay, Staining, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) Spectral library built within the Nuance FX package in order to evaluate the nuclear immunohistochemical expression of the Integrin αvβ6 – raw RGB exemplary image of Integrin αvβ6 immunohistochemistry, as captured by the sensor in RGB mode; (B and C) Correspondent unmixed images showing separate spectra for Hematoxylin, as well as the merged image of DAB and Hematoxylin, clearly show nuclear staining for Integrin αvβ6; (D) Intense reactivity at the level of the tumor stroma, especially in those cases with inflammatory stroma, the lymphocytes and vascular endothelial cells being intensely positive; (E and F) A predominantly weak cytoplasmic reactivity at the level of metastatic proliferations from the lymph nodes. Anti-Integrin αvβ6 immunolabeling: (A–C) ×400; (D and F) ×200; (E) ×100. DAB: 3,3’-Diaminobenzidine; RGB: Red, green and blue

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Immunolabeling

Journal: Communications Chemistry

Article Title: Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors

doi: 10.1038/s42004-024-01144-z

Figure Lengend Snippet: a Engineering of the paper-based electrochemical strip following the screen-printing of conductive ink, modification with a dispersion of gold nanoparticles, covalent engineering of the αvβ6-selective probe and saturation of the surface with mercaptohexanol (6-MCH); b Impedimetric measurements show an increase of the semi-circle (charge transfer resistance, Rct) when the binding between probe and protein occurs (orange line) in comparison with absence of target (black line).

Article Snippet: 25 µg of proteins lysates from cells grown in serum starved condition for 24 h and S-EVs (collected as described in “S-EVs isolation” section) were analysed by SDS-PAGE and Western Blot (WB) performed with antibodies: αvβ6 integrin (1:500 Bioss, bs-5791R), TSG101 (1:500, abcam cat. ab30871), CD81 (1:500, cell signaling #56039), β-actin (1:500, ab30871), γ-tubulin (1:500, sc-17787) Enhanced chemiluminescence (ECL) immunodetection reagents were from GE Healthcare.

Techniques: Stripping Membranes, Modification, Dispersion, Binding Assay, Comparison

Journal: Communications Chemistry

Article Title: Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors

doi: 10.1038/s42004-024-01144-z

Figure Lengend Snippet: a Optimization of the probe length to be immobilized onto the strip. For each experiment each probe at 100 nM concentration has been immobilized onto the strip; the impedimetric measurements have been carried out in presence of 5 ng/mL αvβ6. b Nyquist plots for the nanoengineered biosensor 3 challenged in absence (black dots) and in presence of different concentration of αvβ6, i.e., 1 (cyan dots), 2 (gray dots), 4 (orange dots), 5 (red dots), 10 (green dots) and 20 ng/mL (blue dots). The measurements were carried out as follows: the nanoengineered biosensor was covered with a solution containing the chosen level of αvβ6. After 30 min at room temperature, the biosensors were washed with phosphate buffer, and then covered with a solution containing 1 mM [Fe(CN) 6 ] 3−/4− dissolved in 0.1 M KCl. A potential of 0.2 V and a AC amplitude of 10 mV in a frequency range of 100 kHz to 0.1 Hz have been utilized. The X and Y axes represent, respectively, the real and imaginary components of impedance. The inset i shows the Randles equivalent electrical circuit that has been used to fit the spectra, comprising the electrolyte resistance, Re, in series with a parallel combination of Rct (charge transfer resistance), Zw (diffusion of the analytes in solution and corresponding to Warburg impedance straight line of the curves) and CPE (Constant Phase Element). c Linear range comprised between 1 and 20 ng/mL αvβ6. Inset shows the complete semi-log correlation in a wider range of αvβ6 concentrations, namely 0.01–50 ng/mL.

Article Snippet: 25 µg of proteins lysates from cells grown in serum starved condition for 24 h and S-EVs (collected as described in “S-EVs isolation” section) were analysed by SDS-PAGE and Western Blot (WB) performed with antibodies: αvβ6 integrin (1:500 Bioss, bs-5791R), TSG101 (1:500, abcam cat. ab30871), CD81 (1:500, cell signaling #56039), β-actin (1:500, ab30871), γ-tubulin (1:500, sc-17787) Enhanced chemiluminescence (ECL) immunodetection reagents were from GE Healthcare.

Techniques: Stripping Membranes, Concentration Assay, Diffusion-based Assay

Journal: Communications Chemistry

Article Title: Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors

doi: 10.1038/s42004-024-01144-z

Figure Lengend Snippet: a In presence of 2 ng/mL and ( b ) in presence of 20 ng/mL of αvβ6 (gray bars), α5β1 (green bars) and αvβ3 (orange bars) integrins. The bars are the results of 5 replicates. The dashed red lines indicate the signal observed in absence of targets. The concentrations of integrins have been selected in agreement with the linearity range of the platform.

Article Snippet: 25 µg of proteins lysates from cells grown in serum starved condition for 24 h and S-EVs (collected as described in “S-EVs isolation” section) were analysed by SDS-PAGE and Western Blot (WB) performed with antibodies: αvβ6 integrin (1:500 Bioss, bs-5791R), TSG101 (1:500, abcam cat. ab30871), CD81 (1:500, cell signaling #56039), β-actin (1:500, ab30871), γ-tubulin (1:500, sc-17787) Enhanced chemiluminescence (ECL) immunodetection reagents were from GE Healthcare.

Techniques:

Journal: Communications Chemistry

Article Title: Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors

doi: 10.1038/s42004-024-01144-z

Figure Lengend Snippet: a Representative image of TRPS measurements showing particle size and concentration of S-EVs derived from DU145R80 (R80) cancer cell line (see text). b Upper panels: representative images of Transmission Electron micrograph (TEM) of S-EVs isolated from cell culture media. Magnification 49000X, scale bar 200 nm. The morphology is observed by negative staining. Lower panels: representative images of Immuno Electron micrograph (IEM) of S-EVs, labeled using antibodies specific to αvβ6 integrin and antibody binding was confirmed by Protein A gold-conjugate to 10 nm gold particles. Magnification 120000X, scale bars 100 nm. c Integrin β6 expression by ELISA expressed in terms of fold changes of S-EVs from PC3, A549 and HCT116 compared to R80-derived S-EVs. d Western blot analysis of S-EVs lysates from R80, PC3, HCT116 and A549 and tested with antibodies as indicated in the figure. Both TSG101 and CD81 were tested as positive EVs markers. β actin served as negative control. e Densitometry of Integrin αVβ6/CD81 expression in EVs, expressed as fold changes in either HEK293- or H460- S-EVs compared to PC3-derived S-EVs. Inset shows Western blot analysis of S-EVs lysates from PC3, HEK293 and H460 tested with antibodies as indicated in the figure. Both TSG101 and CD81 were tested as positive EVs markers. ɤ-tubulin served as negative control .

Article Snippet: 25 µg of proteins lysates from cells grown in serum starved condition for 24 h and S-EVs (collected as described in “S-EVs isolation” section) were analysed by SDS-PAGE and Western Blot (WB) performed with antibodies: αvβ6 integrin (1:500 Bioss, bs-5791R), TSG101 (1:500, abcam cat. ab30871), CD81 (1:500, cell signaling #56039), β-actin (1:500, ab30871), γ-tubulin (1:500, sc-17787) Enhanced chemiluminescence (ECL) immunodetection reagents were from GE Healthcare.

Techniques: Concentration Assay, Derivative Assay, Transmission Assay, Isolation, Cell Culture, Negative Staining, Labeling, Binding Assay, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control