integrin alpha v beta 6  (MedChemExpress)

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: (A) Flow cytometric analysis of POD 16-tolerant and CLAD allografts with (A) representative contour plots gated on live CD45-allograft cells (N=5/group) and (B) Intracellular Sftpc expression analysis on CLAD allograft CD326+ αvβ6+ cells. The histogram shown is representative of three independent experiments. (C) Plot of POD 16 allograft numbers of live CD45-CD326+ αvβ6+ cells shown with a mean ± standard deviation from the mean for N=5 per group. Representative immunohistochemical stains with αvβ6 specific antibodies with indicated magnification and scales (N≥4/group).

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Expressing, Standard Deviation, Immunohistochemical staining

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: αvβ6 immunohistological staining of allografts from recipients that underwent club cell injury and received (A) CCL2 neutralizing Abs, (B) CD8α depleting Abs, respectively, and (A, B) Control Ig injections followed by POD 16 [ 64 Cu]Cu-DOTA-A20-K16R PET/CT imaging. Images shown are representative of N≥4 per group. Scale bars: 400 μm for 10x, 100 μm for 40x.

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Staining, Control, Positron Emission Tomography-Computed Tomography, Imaging

Journal: bioRxiv

Article Title: Molecular Imaging of the TGF-β Activating Integrin αvβ6 Detects Chronic Lung Allograft Dysfunction

doi: 10.64898/2026.01.07.698265

Figure Lengend Snippet: (A, Left Panel) Representative autoradiographic images following incubation of [ 64 Cu]Cu-DOTA-A20-K16R with human explanted lung tissue sections from a patient with CLAD and Control lung tissue from a donor lung obtained prior to transplantation. Preincubation with an excess of a cold probe sharply reduces autoradiographic activity (Cold Probe/CLAD). (A, Right Panel) corresponding trichrome-stained lung sections that were incubated with radiotracer. Images are representative of independently conducted experiments, with (B) violin plots showing combined data from 3 explanted CLAD transplants with or without cold-probe blockade and 3 control lungs per group. Two-sided Mann-Whitney U t-test *p<0.05, ***p<0.001. Representative αvβ6 (C) immunohistochemical and (D) immunofluorescent staining of human explanted lungs with CLAD and control lungs (N=3/group). Arrows denote clusters of cells expressing αvβ6. Scale bars: 400 μm for 10x, 100 μm for 40x.

Article Snippet: Sections were then stained with 1:200 αvβ6 antibodies (bs-5791R, Bioss for mouse tissue; AB00891-23.0, Absolute Antibody for human tissue) overnight at 4°C.

Techniques: Incubation, Control, Transplantation Assay, Activity Assay, Staining, MANN-WHITNEY, Immunohistochemical staining, Expressing

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) At the level of striated muscle fibers, where the cytoplasmic pattern predominated; (B) At the level of minor salivary glands, with cytoplasmic and membrane pattern, the reactivity being more evident in the serous acini; (C) At the level of hairs from the lips, with predominant cytoplasmic reactivity; (D) At the level of sebaceous and sweating glands from the lips, with predominant cytoplasmic and nuclear staining (the latter being more evident in the luminal cells of the sweat glands); (E) A weak reactivity observed at the level of the nerve threads; (F) At the level of inflammatory cells (especially in lymphocytes and plasma cells) and in the endothelium of blood vessels, with predominantly cytoplasmic reactivity. Anti-Integrin αvβ6 immunolabeling: (A–F) ×200

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Staining, Clinical Proteomics, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) At the level of normal epithelium, basal cells were not reactive or the cytoplasmic reactivity was weak, but the membrane pattern was especially present at the level of the upper rows of the spinous layer; (B) In low-grade dysplasia, we noticed the tendency to change the subcellular pattern from the membranous to the cytoplasmic to the upper rows of the spinous layer; (C) In moderate-grade dysplasia, the same trend was observed as in low-grade dysplasia, but in the inner two-thirds the reactivity was absent or present with a weak membranous pattern; (D) In high-grade dysplasia, we noticed a nuclear reactivity extended throughout the entire thickness of the epithelium and a weak cytoplasmic reactivity in the upper part of the epithelium; (E) In the epithelium with koilocytic atypia, the reactivity was present in the peripheral cytoplasm, being more evident in the upper layers; (F) In well-differentiated tumors, the prevailing pattern it was membranous especially inside the tumor islands at the level of neoplastic cells with morphology similar to normal keratinocytes. Anti-Integrin αvβ6 immunolabeling: (A–C, E and F) ×200; (D) ×100

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) In well-differentiated OSCC, the ortho- and parakeratotic pearls were negative; (B) In the moderately differentiated OSCC, the predominant reactivity is cytoplasmic, maintaining membrane reactivity only at the level of well-differentiated neoplastic cells; (C) In the less differentiated OSCC, cells lose their membrane reactivity and acquire a predominantly cytoplasmic and even nuclear pattern more evident towards the periphery of the proliferations and in the invasion front; (D) An intense membrane and cytoplasmic reactivity observed at the level of acantholytic neoplastic cells in those variants with extensive foci of acantholysis; (E) In the poorly differentiated OSCC, pattern of reactivity is the same as in moderately differentiated variant with an intensification of the nuclear staining; (F) Regardless of the degree of differentiation, the reactivity was more evident in the invasion front, the invasive islands having a predominantly cytoplasmic and nuclear pattern. Anti-Integrin αvβ6 immunolabeling: (A, C and F) ×100; (B, D and E) ×200. OSCC: Oral squamous cell carcinoma

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Membrane, Variant Assay, Staining, Immunolabeling

Journal: Romanian Journal of Morphology and Embryology

Article Title: Immunoprofile of some surface and cytoplasmic peripheral cell adhesion molecules in oral squamous cell carcinoma

doi: 10.47162/RJME.66.1.17

Figure Lengend Snippet: Integrin αvβ6 immunoreactivity: (A) Spectral library built within the Nuance FX package in order to evaluate the nuclear immunohistochemical expression of the Integrin αvβ6 – raw RGB exemplary image of Integrin αvβ6 immunohistochemistry, as captured by the sensor in RGB mode; (B and C) Correspondent unmixed images showing separate spectra for Hematoxylin, as well as the merged image of DAB and Hematoxylin, clearly show nuclear staining for Integrin αvβ6; (D) Intense reactivity at the level of the tumor stroma, especially in those cases with inflammatory stroma, the lymphocytes and vascular endothelial cells being intensely positive; (E and F) A predominantly weak cytoplasmic reactivity at the level of metastatic proliferations from the lymph nodes. Anti-Integrin αvβ6 immunolabeling: (A–C) ×400; (D and F) ×200; (E) ×100. DAB: 3,3’-Diaminobenzidine; RGB: Red, green and blue

Article Snippet: Then, we incubated the slides, overnight, at 4°C, with the primary antibodies: Integrin αvβ6 (rabbit, polyclonal, 1:150, bs-5791R, Bioss), CD44 (mouse, monoclonal, 1:50, M7082, Agilent) and Ezrin (rabbit, polyclonal, 1:75, 3145, Cell Signaling).

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Immunolabeling